Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: ( A ) Schematic overview of a microarray slide consisting of two blocks. Each block has four identical grids of probes; ( B ) Glass slide during loading procedure of a hybridization mixture; ( C ) Visualization of an hybridized slide after scanning: the green spots indicate the Cy3-Marker spotted at the four corners of the slide; the red spots indicate the positions where a fluorescent fragment of Amphora coffeaeformis SIT gene recognized the complementary oligonucleotide probes; the green square indicates one of the four sectors of one block.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Microarray, Blocking Assay, Hybridization, Marker

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: DNA sequence of the fragments of elongation factor eEF1-a ( A ) and SIT ( B ) obtained by PCR amplification from Amphora coffeaeformis chromosomal DNA. The sense and anti-sense probes specifically designed on this sequence and used for microarray analysis are indicated with black arrows and grey arrows, respectively.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Sequencing, Amplification, Microarray

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: Quantification results of microarray hybridization experiments targeting SIT gene of Amphora coffeaeformis . On the Y-axis are reported the probes producing a fluorescent signal after hybridization: yellow bars correspond to probes displaying high specificity, green bars indicate probes with lower specificity, while gray bars correspond to probes producing false-positive signals. The fluorescence intensity signals are reported on the histogram bars.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Microarray, Hybridization, Fluorescence

Journal: Gene Regulation and Systems Biology

Article Title: Intensity Dependent Confidence Intervals on Microarray Measurements of Differentially Expressed Genes: A Case Study of the Effect of MK5, FKRP and TAF4 on the Transcriptome

doi:

Figure Lengend Snippet: Overview of the different experiments.

Article Snippet: Microarray , KTH Rat27K Oligo m.a, OperonV3.0 Tecan HS 4800 Genepix 4000B Genepix Pro 6.1.0.2 , , , Applied Biosystems Mouse Genome Survey m.a.V2.0 DIG Labeling Applied Biosystems 1700 scanner , , , Applied Biosystems Human Genome Surveym. aV2.0 DIG Labeling Applied Biosystems 1700 scanner , , , .

Techniques: Knockdown, Labeling, Microarray, Control

Journal:

Article Title: Large-scale analysis of DNA methylation in chronic lymphocytic leukemia

doi: 10.2217/epi.09.10

Figure Lengend Snippet: This cluster is based on a 10% threshold where there are 12 samples with less than 10% of the cells expressing CD38 and 26 samples with more than 10% CD38 expression. This illustrates a measure of relatedness of DNA methylation across all loci for each sample. Each column represents a patient sample and each row represents a clone/locus on microarray chip. The florescence ratios of cy3/cy5 are measures of DNA methylation and are depicted as a color intensity (-0.5 to +0.5) in log base 2; yellow indicates loci that have a higher level of DNA methylation in chronic lymphocytic leukemia compared with normal controls, blue indicates a lower level of methylation and black indicates no change. Graded colors across the spectrum represent various levels of methylation. The dendrogram from the top of the cluster (rotated 90° and enlarged on the right) represents the CD38 expression level of each sample. Not every patient sample clustered with the expected group. The numbers shown in blue color are seven patients with CD38high that are clustered in the group of 10% or less, and one sample with 6% CD38 clustered with the CD38high group.

Article Snippet: The slides were processed after hybridization using the Pronto Universal Microarray Reagents (Corning Life Science, MA, USA).

Techniques: Expressing, DNA Methylation Assay, Microarray, Methylation